In this study we have used different methods for the
preparation of liposomes to encapsulate antioxidant molecules and
have determined whether they retain their antioxidant activities.
We have compared different methods and determined the most
suitable method for preparation of liposomes containing watersoluble
and lipid-soluble antioxidants.
Materials and Methods: Different preparative techniques
such as thin-film hydration, reverse-phase evaporation, freezethaw
and periodic sonication were used to produce liposomes.
These methods were used to encapsulate different antioxidant
molecules and were compared for antioxidant activity. Light
microscopy, fluorescence microscopy and particle size analyzers
were used in order to characterize the prepared liposomes and to
analyze their particle size, distribution and shape.
Results: Liposomes containing water-soluble antioxidants
such as vitamin C and polyphenols and fat-soluble antioxidant
vitamin E were prepared. The separation of free molecules from
those that were liposomed was performed with Sephadex G-25 gel
filtration chromatography. The antioxidant activity of vitamin E
liposomes was highest in comparison to the others.
Conclusion: Among the methods we have used for preparing
liposomes sonication is the fastest and also most advantageous
because organic solvents such as methanol or chloroform are not
used during preparation. Both water-soluble antioxidant molecules
and lipid-soluble antioxidants could be prepared with this method
and preserved their activities.