2003, Cilt 16, Sayı 1, Sayfa(lar) 007-011
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Berna K. Oktar1, Meral Yüksel2, İnci Alican1
1Department of Physiology, School of Medicine, Marmara University, İstanbul, Turkey
2Department of Biochemistry, School of Medicine, Marmara University, İstanbul, Turkey
Keywords: α-melanocyte-stimulating hormone, Burn, Reactive oxygen species, Nitric oxide, Chemiluminescence
Objective: Previous findings related to the anti-inflammatory effects of α-melanocyte stimulating hormone (α-MSH) indicate that the peptide might inhibit inflammation by antagonizing the effects of local cytokines or by inhibiting neutrophil functions. In addition, it is a potent inhibitor of the induction of the inducible nitric oxide synthase (iNOS) in cultured macrophages and of nitric oxide (NO) production in a sepsis model. This study was designed to investigate whether α-MSH affects reactive oxygen metabolite production by rat peritoneal neutrophils following burn injury and whether NO is involved in this effect.

Methods: The neutrophils were removed from the peritoneum of the rats 6 h after burn or sham trauma. Cells (2 X 106 /ml) were treated with the increasing concentrations of α-MSH (10–12 M, 10–10 M, 10–8 M or 10–6 M). Nitric oxide donor, sodium nitroprusside ranging from (10–10 - 10–4 M) was added to the cells in the presence of α-MSH. Reactive oxygen metabolite production was measured by chemiluminescence (CL) technique using luminol or lucigenin probes.

Results: Lucigenin CL value of cells obtained from burn animals was significantly higher than those obtained from the sham group. α-MSH caused significant reductions in high lucigenin CL values and this effect was partly inhibited by sodium nitroprusside.

Conclusion: Our results suggest that α-MSH is effective in preventing oxidant production by neutrophils activated by burn trauma, at least in part, by a mechanism involving nitric oxide.

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