2003, Cilt 16, Sayı 1, Sayfa(lar) 007-011
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THE EFFECT OF α-MELANOCYTE STIMULATING HORMONE ON BURN-INDUCED OXIDANT PRODUCTION BY RAT PERITONEAL NEUTROPHILS
Berna K. Oktar1, Meral Yüksel2, İnci Alican1
1Department of Physiology, School of Medicine, Marmara University, İstanbul, Turkey
2Department of Biochemistry, School of Medicine, Marmara University, İstanbul, Turkey
Keywords: α-melanocyte-stimulating hormone, Burn, Reactive oxygen species, Nitric oxide, Chemiluminescence
Abstract
Objective: Previous findings related to the anti-inflammatory effects of α-melanocyte stimulating hormone (α-MSH) indicate that the peptide might inhibit inflammation by antagonizing the effects of local cytokines or by inhibiting neutrophil functions. In addition, it is a potent inhibitor of the induction of the inducible nitric oxide synthase (iNOS) in cultured macrophages and of nitric oxide (NO) production in a sepsis model. This study was designed to investigate whether α-MSH affects reactive oxygen metabolite production by rat peritoneal neutrophils following burn injury and whether NO is involved in this effect.

Methods: The neutrophils were removed from the peritoneum of the rats 6 h after burn or sham trauma. Cells (2 X 106 /ml) were treated with the increasing concentrations of α-MSH (10–12 M, 10–10 M, 10–8 M or 10–6 M). Nitric oxide donor, sodium nitroprusside ranging from (10–10 - 10–4 M) was added to the cells in the presence of α-MSH. Reactive oxygen metabolite production was measured by chemiluminescence (CL) technique using luminol or lucigenin probes.

Results: Lucigenin CL value of cells obtained from burn animals was significantly higher than those obtained from the sham group. α-MSH caused significant reductions in high lucigenin CL values and this effect was partly inhibited by sodium nitroprusside.

Conclusion: Our results suggest that α-MSH is effective in preventing oxidant production by neutrophils activated by burn trauma, at least in part, by a mechanism involving nitric oxide.

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