2014, Cilt 27, Sayı 3, Sayfa(lar) 166-170
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Preparation of liposomes containing vitamin C, vitamin E and polyphenol extract and comparison of their antioxidant activities
Ziba Mokhberi OSKOUEI, Gökhan BİÇİM, A. Suha YALÇIN
Tıbbi Biyokimya Anabilim Dalı, Tıp Fakültesi, Marmara Üniversitesi, Başıbüyük, Maltepe-İstanbul, Türkiye
Anahtar Kelimeler: Antioxidant liposome, Vitamin C, Vitamin E, Polyphenols

Objective: In this study we have used different methods for the preparation of liposomes to encapsulate antioxidant molecules and have determined whether they retain their antioxidant activities. We have compared different methods and determined the most suitable method for preparation of liposomes containing watersoluble and lipid-soluble antioxidants.

Materials and Methods: Different preparative techniques such as thin-film hydration, reverse-phase evaporation, freezethaw and periodic sonication were used to produce liposomes. These methods were used to encapsulate different antioxidant molecules and were compared for antioxidant activity. Light microscopy, fluorescence microscopy and particle size analyzers were used in order to characterize the prepared liposomes and to analyze their particle size, distribution and shape.

Results: Liposomes containing water-soluble antioxidants such as vitamin C and polyphenols and fat-soluble antioxidant vitamin E were prepared. The separation of free molecules from those that were liposomed was performed with Sephadex G-25 gel filtration chromatography. The antioxidant activity of vitamin E liposomes was highest in comparison to the others.

Conclusion: Among the methods we have used for preparing liposomes sonication is the fastest and also most advantageous because organic solvents such as methanol or chloroform are not used during preparation. Both water-soluble antioxidant molecules and lipid-soluble antioxidants could be prepared with this method and preserved their activities.

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